Scholarship of Discovery
We streamlined a protocol for isolating chromosomal DNA from buccal cells for the purpose of producing restriction fragment length polymorphisms (RFLPs) and identifying ABO genotype. This protocol involves DNA isolation, polymerase chain reaction, restriction enzyme digestion, and gel electrophoresis. Buccal cells are a viable source of chromosomal DNA for amplifying the ABO gene segments of interest. The collection of buccal cell samples is a less invasive method than collecting blood samples, which requires venipuncture. The DNA isolated from buccal cells was comparable in quality to the DNA isolated from blood cells. A complete protocol was produced for use in an undergraduate level laboratory.
Ginn, Brian M., "Genetic Determination of ABO Genotype from Buccal Cells: Incorporating PCR, Gel Electrophoresis, and ABO Genotyping into Undergraduate Study" (2014). Honors Program Projects. 55.