Document Type

Article

Peer Reviewed

1

Publication Date

3-2014

Scholarship Domain(s)

Scholarship of Discovery

Abstract

We streamlined a protocol for isolating chromosomal DNA from buccal cells for the purpose of producing restriction fragment length polymorphisms (RFLPs) and identifying ABO genotype. This protocol involves DNA isolation, polymerase chain reaction, restriction enzyme digestion, and gel electrophoresis. Buccal cells are a viable source of chromosomal DNA for amplifying the ABO gene segments of interest. The collection of buccal cell samples is a less invasive method than collecting blood samples, which requires venipuncture. The DNA isolated from buccal cells was comparable in quality to the DNA isolated from blood cells. A complete protocol was produced for use in an undergraduate level laboratory.

Comments

Honors Capstone Project completed in 2014 for Olivet Nazarene University.

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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