Document Type
Article
Peer Reviewed
1
Publication Date
3-2014
Scholarship Domain(s)
Scholarship of Discovery
Abstract
We streamlined a protocol for isolating chromosomal DNA from buccal cells for the purpose of producing restriction fragment length polymorphisms (RFLPs) and identifying ABO genotype. This protocol involves DNA isolation, polymerase chain reaction, restriction enzyme digestion, and gel electrophoresis. Buccal cells are a viable source of chromosomal DNA for amplifying the ABO gene segments of interest. The collection of buccal cell samples is a less invasive method than collecting blood samples, which requires venipuncture. The DNA isolated from buccal cells was comparable in quality to the DNA isolated from blood cells. A complete protocol was produced for use in an undergraduate level laboratory.
Recommended Citation
Ginn, Brian M., "Genetic Determination of ABO Genotype from Buccal Cells: Incorporating PCR, Gel Electrophoresis, and ABO Genotyping into Undergraduate Study" (2014). Honors Program Projects. 55.
https://digitalcommons.olivet.edu/honr_proj/55
Included in
Biology Commons, Genetics Commons, Molecular Genetics Commons
Comments
Honors Capstone Project completed in 2014 for Olivet Nazarene University.